Testing
Lab Note #3 The result Experiment date: 2020. 8. 31 Material: Microscope, cell counting machine, the result of the first experiment 1. Observing the result of the last experiment Through a microscope, I observed colorectal cells that were treated with PLA2 and 5-FU. Then I took pictures of them through a computer connected to the microscope. Though I expected to observe the reduced cells, I was surprised to see the cells that reduced only in a small amount. So I decided to use cell counting machine to get the exact result. 2. Counting cells Dead cells are stained red while alive cells are stained green. The machine then count the number of green cells and red cells. First, I put small amount of buffer solution with chemical (PLA2 or 5FU) and cells on the small plate. (Pipetting should be done in order to make sure that cells aren't compacted together.) Then, I put the plate on the machine which starts counting. After I check the result, I saved it on the U...
Comments
Post a Comment