Skip to main content

Lab Note #3 The result

 

Lab Note #3 The result

Experiment date: 2020. 8. 31

Material: Microscope, cell counting machine, the result of the first experiment

1. Observing the result of the last experiment 

Through a microscope, I observed colorectal cells that were treated with PLA2 and 5-FU. Then I took pictures of them through a computer connected to the microscope. 

Though I expected to observe the reduced cells, I was surprised to see the cells that reduced only in a small amount. So I decided to use cell counting machine to get the exact result. 

2. Counting cells

Dead cells are stained red while alive cells are stained green. The machine then count the number of green cells and red cells. 

First, I put small amount of buffer solution with chemical (PLA2 or 5FU) and cells on the small plate. (Pipetting should be done in order to make sure that cells aren't compacted together.) Then, I put the plate on the machine which starts counting. After I check the result, I saved it on the USB. Though the procedure seemed simple, I had to pay attention since it is easy to count the same solution twice. 

The result wasn't clear as I thought although I consider error. To be more specific, I expected to observe cells that reduce as the concentration of PLA2 and 5FU increase, but I could observe some cells that increased though the concentration of chemical increased as well. I thought it was because I made a mistake during the experiment. 

<Result>


Labels:

Comments

Post a Comment

Popular posts from this blog

Now I know.

 A grim-faced girl who is waving her hands violently to get a taxi on streets. Her red face was soaked with tears continuously falling down. Because she couldn't breathe properly, she had to keep making weird noise trying to breathe. Everybody passing her by was glancing at her wondering what is wrong with her. Some of them might have wondered whether they should help her, but they could not get close to her easily because she seemed to be out of mind. She seemed literally crazy.   This was how I looked when I heard that dad collapsed and was rushed to the emergency room from mom on the phone. Because she told me that I should keep it secret from my little sister, I had to suppress my surprise in house and burst into tears on streets, getting taxi to go to a hospital dad's in.   "God, please secure him."   In a taxi, I kept praying to god wishing him to be safe. I wished it was a slight sickness.   "Mom! Is he okay? Can I see him?"  I as...
Post a Comment
Read more

Why you should...

Speech 2 Prompt: Why you should.... Come up with a title using the above, and state it at the beginning of your speech. Keep in mind that this is basically open topic, and can be either a warning against something or a positive piece of advice. I suggested KMLA, but you mostly voted to make it open. My advice: Write it out completely and follow a Journey/Character Arc and segment it into a Memory Palace with CLEARLY defined segments. Requirments: You must use ONE MEANGINGFUL PAUSE in your speech. I will demonstrate this. You must make use of at least one segment of DIALOGUE.0  You must use the expression - "For the love of god" one time. 0 You must use REPETITION at least once. Ex: "Maybe it was because the weather was rainy. Maybe it was because my shoes were wet. Maybe it was because my umbrella was broken. But clearly, the weather made my mood only worse, so I screamed at my brother." 0  Have fun, don't be afraid to read from a paper if you aren't fully c...
Post a Comment
Read more

U island

Is there any place that you can't go again even if you want to? I have. U island - a place where I saw hundreds of blackfaced spoonbills.  U island prohibits public access because of its location that is so close to North Korea (A soldier told us that one soldier escaped from North Korea and came here through U island in the past) It was lucky for our DMZ exploration team got a chance to look through it for exploration purposes. (The government might have allowed us to explore it because CNN was filming DMZ, exploring it with us.) After we heard precautions from soldiers, such as 'don't take any pictures and upload it' or 'Don't go too close to a barbed-wine fence', we went into a small guard post where we can see U island at a glance. As soon as we finished installing telescope and adjusting its focus, we started to find blackfaced spoonbills, and I couldn't stop overusing exclamation. Can you guess why? We didn't have to 'find' them, but ha...
Post a Comment
Read more